芦苇植株再生体系优化

王子岚,高品红,魏新燕,李燕玲,杜克久

林业与生态科学 ›› 2012, Vol. 27 ›› Issue (3) : 313-315.

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林业与生态科学 ›› 2012, Vol. 27 ›› Issue (3) : 313-315.

芦苇植株再生体系优化

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Establishment and optimization of the plantlet regeneration systemof Phragmites australis(Cav.)Trin.ex Steud

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摘要

在前人工作基础上利用禾本科芦苇成熟种子为外植体建立了植株再生体系。芦苇成熟种子经2%洗涤灵溶液洗涤,70%乙醇处理30s,20%“84”消毒液处理25min后接种到MS+4%蔗糖(pH 5.5)的种子萌发培养基中培养,种子萌发率为62%。萌发种子继代至MS+1mg/L 6-BA+0.1mg/L 2,4-D+0.5mg/LNAA+4%蔗糖(pH 5.5)培养基进行不定芽诱导,不定芽发生率为95%。无根丛生芽继代到不定根诱导培养基1/2MS+0.5mg/L IBA +0.5mg/L NAA+4%蔗糖(pH 5.5),不定根发生率为90%。

Abstract

The mature seeds were used as explants to establish plant regeneration system ofPhragmites australis(Cav.)Trin.ex Steud based on the published information.The seedswhen treated with 2% detergent,70%ethanol for 30s,20% “84”disinfectant for 25min,and inoculated into MS+4%sucrose(pH 5.5)medium,showed a 62% germination rate.When the germinated seeds were subcultured into MS+1mg/L 6-BA+0.1mg/L 2,4-D+0.5mg/L NAA+4%sucrose(pH 5.5)medium,the 95%adventitious bud induction rateappeared.The 90%adventitious root formation rate was obtained with the adventitious rootinduction medium of 1/2MS+0.5mg/L IBA +0.5mg/L NAA+4%sucrose,pH 5.5.

关键词

芦苇 / 成熟种子 / 植株再生

Key words

Phragmites australis(Cav.)Trin.ex Steud / mature seeds / plant regeneration

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导出引用
王子岚,高品红,魏新燕,李燕玲,杜克久. 芦苇植株再生体系优化[J]. 林业与生态科学, 2012, 27(3): 313-315
WANG Zi-lan,GAO Pin-hong,WEI Xin-yan,LI Yan-ling,DU Ke-jiu. Establishment and optimization of the plantlet regeneration systemof Phragmites australis(Cav.)Trin.ex Steud[J]. Forestry and Ecological Sciences, 2012, 27(3): 313-315

基金

中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室开放基金(KF2009-03)。
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